FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48116756
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT534830.11115254735793079No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG27750.023.917976
ACCGCGT122100.021.6699056
GACCGCG123650.021.4164585
TCGACGA32450.021.0084137
CGGTATA45050.020.92591920
AGACCGC128250.020.7535634
CCGCGTT130100.020.4411587
CAGACCG133100.020.2508873
CGCGTTC132350.020.0936498
GAACGAT138550.019.51925545
CGACGAG35650.017.8604418
AATACGG154900.017.2413932
ATACGGA160100.016.79382133
TACGGAG160350.016.54313734
TTCGGCG79350.016.33205644
TCGGCGC78800.016.3318445
TCAGACC171700.016.2224222
AACGCTA114450.015.94301323
ACGCTAC114750.015.920938524
AGAACGA171300.015.89254144
Produced by FastQC (version 0.11.5)