FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48116756
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAG101000.013.81145235
AGCTTAG107100.013.23357645
CATAGGG109000.012.96295337
ATAGGGG113850.012.7480738
GCATAGG113650.012.31363736
GAGCTTA125750.011.45160344
TCAGACG75900.010.8522162
GTCACTC161800.010.6930478
TGGCATA133400.010.65933834
GGGAGGT205950.010.0520283
ACCCGGC74600.010.0435565
GGTCACT171350.010.0312757
AGGTCAC185300.09.7010396
GTTGTCG24000.09.46760826
ACTCTCC193000.09.46562311
TAATCGG38700.09.3027424
CGTCGAT18300.09.22062212
ATAGCTC77900.09.15660739
TAGCGTA10400.09.08629337
GGAGGTC207100.09.0612454
Produced by FastQC (version 0.11.5)