FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52904324
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG30550.013.62444226
AGCTTAG153450.011.53911945
ATAGGGG164400.011.13998338
GGCATAG164000.010.93410935
GGGAGGT256400.010.9013143
GGAGGTC243400.010.540394
GAGCTTA185700.09.74102944
TGTCGTT46100.09.70983428
ATATAGC50550.09.6591636
GGTCACT260950.09.4936537
CATAGGG192400.09.46046237
TGGCATA196200.09.26554634
GTCGTTC50950.09.00600329
GTCACTC299300.08.87904558
GCATAGG206450.08.84936436
ACTCTCC292950.08.73277511
ACCCGGC65400.08.5337235
TATAGCT56350.08.30563457
CGTTCCC52550.08.17590631
GCTAAGT52000.08.13430614
Produced by FastQC (version 0.11.5)