FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51353272
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG565650.11014877494076718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAG185100.015.40925435
ATAGGGG190250.015.15658338
AGCTTAG187950.015.1038845
GGGAGGT317800.014.3319473
GGAGGTC309600.013.9624954
GAGCTTA214000.013.52724744
TGGCATA217000.013.37237534
CATAGGG216200.013.31679837
GTCACTC324750.012.8978018
GGTCACT320650.012.894767
ACTCTCC325100.012.8684511
GTTGTCG23700.012.34046526
GCATAGG235300.012.2456236
TAGGGGA371300.011.24292939
AGGTCAC384900.011.1868646
CACTCTC380050.011.10291610
GAGGTCA398050.011.0904775
CGTAGGG117900.010.87443237
GCGTAGG117800.010.76929836
GGCGTAG119750.010.68822435
Produced by FastQC (version 0.11.5)