FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40891140
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT31950.019.4484541
TTGCGTC68850.019.08453843
CCCGTAT34950.017.0060561
CTCGGTT88500.016.8661961
AACGCTA100950.015.98030523
ACGCTAC103100.015.75617424
CTACACG41600.015.63060145
CGCTACC105150.015.36338325
CTCCGAT43800.015.06053451
CGTACGC57850.014.9354985
CCGTACG59500.014.8616644
TTCGGCG58050.014.53450444
CCCGGAT92650.014.3125411
ACGGTAC50650.013.99272445
AAAACGC117300.013.96386221
CTTGCGT94450.013.88798842
GCCGGCT125150.013.7079011
TCAATCG98100.013.6464338
TCACGCT125700.013.6392618
CTCCGGT55000.013.5900881
Produced by FastQC (version 0.11.5)