FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40891140
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG100000.014.283945
ATAGGGG109750.012.9739538
CATAGGG114650.012.45869937
GGCATAG110750.012.45061735
GACGCAA61550.012.38924144
GAGCTTA120350.011.98077744
GTCACTC154600.011.7339878
GGTCACT157000.011.4685997
ACCCGGC48950.011.3557415
ACGCAAG69050.011.30417545
GCATAGG126150.011.18029736
TAGCGTA6850.011.16506937
TGGCATA127000.011.15863234
TCAGACG72550.011.1047382
GGGAGGT182700.010.8640873
ACTCTCC171550.010.5615311
GGAGGTC182150.010.5016814
AGGTCAC181850.010.2108226
GAGGTCA195550.09.9432225
ATAGCGT8550.09.47129736
Produced by FastQC (version 0.11.5)