FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50016192
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA120700.018.09173423
ACGCTAC121300.017.87292124
CGCTACC124900.017.30393625
ACGTTCG16700.016.7772736
AAAACGC135350.016.2322621
TCACGCT144700.016.016958
CCCGTAT32400.015.434581
GAAAACG150750.014.70711720
CCGGCTT184700.014.4545732
GCCGGCT184250.014.361821
GTCCGTT45350.014.2907661
AAACGCT156250.014.26230422
TTCACGC165000.014.0599647
GTCGGTT45650.013.6073631
AGACCGC50300.013.5029864
TTCGACG14150.012.98928456
ACGCTCA162900.012.934051510
CCCGGAT139400.012.9338621
GTTTTCG172400.012.67372645
GCGGACT72450.012.59262852
Produced by FastQC (version 0.11.5)