FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50016192
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG171500.017.09150345
ATAGGGG207850.014.41883738
GGCATAG208200.014.33004935
GAGCTTA209400.014.26664744
GGGAGGT333150.013.3896423
GTCACTC321450.013.3298458
GGTCACT320000.013.2860017
TGGCATA239000.012.61356934
GGAGGTC350950.012.6025174
GAGGTCA353050.012.4829325
AGGTCAC353750.012.4081816
ACTCTCC348800.012.29083611
CATAGGG255250.011.84585437
GCATAGG263050.011.42659136
TCACTCT386300.011.25329
GTTGTCG25400.010.85953326
CACTCTC399250.010.67644710
TGGGAGG309000.010.3832612
TAGGGGA410750.010.22673739
AGCATAG121400.010.16511945
Produced by FastQC (version 0.11.5)