FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61805785
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1746980.2826563888801024No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG1304850.21112101399569635No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG812790.1315071073039522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG60700.017.27290526
AGCTTAG286200.015.56616545
GGGAGGT396400.015.2694853
ATATAGC78050.014.9899816
GGAGGTC395200.014.6952324
GGCATAG316750.014.18500935
GTCGTTC74500.014.0733629
GTCACTC406950.013.9715628
GCTAAGT78600.013.7125114
AACTCTA144350.013.68559945
ATAGGGG347700.013.61473538
GAGCTTA329900.013.511023544
GGTCACT426500.013.3784387
ACTCTCC435200.012.92022611
TGTCGTT82900.012.7287728
GAGGTCA466450.012.6242055
TGGCATA360950.012.54148934
TTGTCGT87950.012.53511627
CACCGAA753850.012.49396845
AGGTCAC472850.012.4618566
Produced by FastQC (version 0.11.5)