FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54072604
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1931160.357142038138204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT324150.028.97234245
AGAACGA392550.023.95270744
ACCGCGT408150.023.2858126
GACCGCG410500.023.1030045
AGACCGC412850.023.0477984
CCGCGTT415300.022.8848067
CGCGTTC416050.022.8597778
CAGACCG440350.021.7256153
AATACGG453650.020.71659532
TCAGACC467850.020.640962
ATACGGA455650.020.61578633
TACGGAG459600.020.41902434
GCGTTCT470850.020.2756949
AAGAACG469450.020.1057343
CAATACG482350.019.50727331
ACGGAGA492850.019.0779835
CTCAGAC552400.018.7288551
CGGAGAG515150.018.26522836
CCCAATA522700.018.1262529
CCCCAAT537050.017.67127428
Produced by FastQC (version 0.11.5)