FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61971319
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG25700.019.606956
GCCGGCT233100.019.4264281
CCGGCTT260850.018.6297992
AACGCTA234800.018.44854223
ACGCTAC235600.018.3190424
TCACGCT244200.018.1844658
TTCACGC260800.017.1131237
ACGCTCA260850.016.94613810
CGCTACC200400.016.59611725
AAAACGC270150.016.23404721
CCCGTAT40950.016.2134461
GAAAACG244450.016.08041420
CGGCTTC321300.016.0335143
CACGCTC290400.015.4154259
CGCTACT66400.015.25015425
ACGTTCG16200.015.1358466
TCGACGA35700.015.1230097
AAACGCT290700.015.04806422
CGTGGTT290150.014.74657541
GTCCGTT44750.014.6857811
Produced by FastQC (version 0.11.5)