FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53079353
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4325140.8148441447656682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG33850.013.15959626
ACCCGGC90750.011.8018485
TCACCCG106650.010.7608083
CGGACAC97300.010.52221813
ACGGACA110300.09.97472118
ACACGGA104250.09.92877916
CCGGACA106350.09.92283612
TGTCGTT46050.09.77096128
ATAGCTC108200.09.62705639
GCCCGGA115950.09.62516310
CACCGAA219350.09.56150445
CGGACAG106900.09.47118519
CACCCGG124250.09.4163874
GACACGG112200.09.3053315
GCGTTCG22550.09.18011115
GCACCGA228500.09.12947944
ACCCGGG111700.09.0856772
CGGCCCG120000.09.0750298
GCGTAGG120600.09.04839136
CGTAGGG121000.09.03635237
Produced by FastQC (version 0.11.5)