FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46234303
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA477920.10336913698039311No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG55300.028.6014426
TCGACGA63600.025.2574777
CGACGAG62200.024.8494138
GTTCGAC66050.024.0487735
CGGTATA94200.023.716820
AGTTCGA79300.020.3709984
ATGACGG109450.019.81615816
AACGACG35300.019.6306316
CAGTTCG88350.019.252023
CGAGGAT94600.019.09754211
GACGAGG101100.018.7152489
ATAGAGT132900.017.69302724
GTTCCTA108450.017.61308515
GACGGTA134150.016.9055218
CGGTATG54700.016.82346221
CCGGCTT182900.016.7788312
CCCGTAT30150.016.6557921
GTATAGA143500.016.5418922
GATGACG131900.016.06805815
TCGTATG33550.016.0270943
Produced by FastQC (version 0.11.5)