FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46234303
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG569020.12307312170359744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG35250.015.51035426
GGAGGTC273950.014.7768174
GGGAGGT296850.014.57622053
AACTCTA126650.014.54795845
GCGTCGT37200.014.397264511
AGCTTAG230900.014.0398845
GGCATAG236900.013.66560735
TCGTGTA39850.013.38338514
GGTCGCC39850.013.21008843
ATAGGGG255150.013.11973438
GTCACTC301300.013.0620778
GGTCACT305100.012.9730587
ATATAGC49750.012.754976
ACTCTCC310500.012.61053811
GAGCTTA262400.012.33723844
TGGCATA271850.011.93372934
CATGCAT97950.011.8085399
GCTAAGT48750.011.7247914
TATATAC64400.011.6702277
GTCGTGT45650.011.53509113
Produced by FastQC (version 0.11.5)