FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53064640
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA696180.13119470894365814No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG570800.1075669221538109No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAG169400.014.80632635
ATAGGGG179650.014.34798438
AGCTTAG176350.014.13468745
GTTGTCG36450.013.70472226
GGTCACT257200.013.6234197
CATAGGG188900.013.31227637
GTCACTC268300.013.2357278
ACTCTCC274500.013.08370511
AGGTCAC281800.012.7937726
TGGCATA199050.012.78185934
GCATAGG199500.012.63892436
AGGGATC164850.012.6340472
GAGCTTA201500.012.56841344
GGGAGGT306150.012.4936883
GGAGGTC296300.012.39193254
GAGGTCA303750.012.1829935
TGTCGTT44500.011.075128
CGTAGGG86850.011.00681137
ACCCGGC83050.011.00410655
GCGTAGG87050.010.98164836
Produced by FastQC (version 0.11.5)