FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50014965
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA536020.10717192344331342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG36000.014.87255926
GGCATAG131250.011.82958735
CACCGAA239800.011.788815545
AGCTTAG135650.011.6976645
TGTCGTT46200.011.68639328
ATAGGGG137300.011.48971938
GCACCGA250300.011.25836644
CATAGGG148150.010.51097337
GAGCTTA149750.010.40090644
GGGAGGT217550.010.3523453
GGAGGTC211900.010.2142834
GCTAAGT53100.010.21142414
TCTATAG287350.010.0848263
GCATAGG154450.010.08190836
ATATAGC59150.010.0795076
TGGCATA161700.09.9637334
AACTCTA120800.09.85640245
CTATAGT296650.09.8065894
GTCACTC209600.09.7677068
TATAGCT65550.09.713197
Produced by FastQC (version 0.11.5)