FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50460874
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2578940.5110771565312167No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG639640.1267595959594358No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA582770.11548947804590147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT419150.030.04096245
AGAACGA492150.025.62980544
ACCGCGT511900.024.6892916
AGACCGC515250.024.677194
GACCGCG516250.024.4943285
CAGACCG523650.024.3199963
CCGCGTT522150.024.2003237
CGCGTTC531100.023.796748
TCAGACC559950.023.1531142
AAGAACG573900.021.96862843
GCGTTCT579550.021.888749
ATACGGA578850.021.87628733
AATACGG580300.021.85735532
TACGGAG581000.021.75154134
CTCAGAC638950.021.1669851
CAATACG610900.020.8227131
ACGGAGA608300.020.75972735
CCCAATA627450.020.37610829
CGGAGAG624250.020.07956336
CCAATAC655700.019.58031530
Produced by FastQC (version 0.11.5)