FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50460874
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA513070.10167679616488608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG169750.014.31720345
GTTGTCG27000.013.25125126
GGCATAG189050.012.87732935
GTCACTC257450.012.738078
ATAGGGG199600.012.45484138
GGTCACT259750.012.417647
ACCCGGC95000.012.0281655
GAGCTTA207750.011.83720544
CATAGGG209400.011.73317637
GGGAGGT322600.011.6069613
TGGCATA216400.011.3746934
ACTCTCC289150.011.30436211
AGGTCAC304400.011.1801146
GAGGTCA314700.011.0216585
GCATAGG222850.010.99468236
GGAGGTC313350.010.803554
ATAGCTC98800.010.771173539
CGGACAC105900.010.73110313
TCACCCG109400.010.6958563
TGTCGTT35650.010.28840728
Produced by FastQC (version 0.11.5)