FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54077048
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG29600.022.6436066
ACCGCGT63100.021.4223046
AGACCGC68100.019.9719264
TCGACGA34650.019.4083677
CCGCGTT73450.018.3730187
GACCGCG74200.018.2389875
TGCGCGT45150.018.18220145
TCGGCGC88650.018.0626945
CGGTATA54150.018.02875320
ATGCGCG44200.018.01776144
CAGACCG80150.017.4771753
TTCGGCG94050.017.07887544
CGTACGC101050.016.1113575
CGCGTTT26350.015.7910918
CGACGAG41900.015.7817218
CCGTACG102800.015.7713734
CTACACG55950.015.55929945
TACGCCA109100.015.2761717
CCCGTAT28350.015.25956251
ACGTTCG30000.014.9944256
Produced by FastQC (version 0.11.5)