FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52988639
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1372530.2590234484037229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT288050.024.18287345
ACCGCGT309350.022.5176376
AGACCGC314450.022.2597544
GACCGCG313200.022.2192885
TTCGACG26450.022.2017296
CCGCGTT319100.021.8506667
CAGACCG326700.021.5353933
CGCGTTC323700.021.5257668
AGAACGA350000.020.00517344
TCAGACC365100.019.8389022
ATACGGA367350.018.9384233
GCGTTCT369200.018.9292169
AATACGG369550.018.82567632
TACGGAG369000.018.77446634
CTCAGAC444350.017.8238581
AAGAACG395700.017.7402543
CAATACG396200.017.70070831
ACGGAGA405800.017.13843735
TCGACGA36150.017.1156867
CGGTATA42050.017.0158820
Produced by FastQC (version 0.11.5)