FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52988639
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG33100.010.87279426
ACCCGGC82550.010.4975635
TGTCGTT37600.09.92941228
ATATAGC46600.09.8034996
AGCTTAG120000.09.76796445
ATAGCTC83300.09.643929539
ATAGGGG126400.09.63121338
ACGGACA86450.09.63019718
GGCATAG122650.09.53890535
TCAGACG102950.09.5098592
CGGACAC91900.09.08341213
TAGCTCT87550.08.99589440
TAATCGG41150.08.6423954
GTCGTTC43650.08.60455729
TATAGCT49500.08.5926597
GGGAGGT225850.08.5703343
CATAGGG140000.08.56564937
TCACCCG104450.08.5330873
AGGGATC153900.08.4235482
CAGACGT117800.08.3685123
Produced by FastQC (version 0.11.5)