FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54651166
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG163750.013.8739845
GTTGTCG37500.013.00255226
GGCATAG179050.012.77250535
ACCCGGC90250.012.7179045
ATAGGGG186250.012.38061538
GTCACTC267000.011.8434338
TCACCCG101850.011.6233793
GAGCTTA198600.011.60083444
GGTCACT273250.011.4987897
GGGAGGT317900.011.4191473
CATAGGG204200.011.18714337
ACGGACA104600.010.93246818
ACTCTCC289250.010.817211
GGAGGTC302050.010.8061074
TGGCATA216050.010.64305234
GCATAGG217800.010.52833936
AGGTCAC309950.010.5209186
TGTCGTT46600.010.49929328
CGGACAG109900.010.18069219
ATAGCTC106650.010.13373339
Produced by FastQC (version 0.11.5)