FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47537263
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG719840.15142647148196142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG28800.023.0469536
TCGACGA34850.019.7556677
CGGTATA44750.018.80391920
GTTCGAC36200.018.3989565
GCCGGCT217000.017.7212581
CCGGCTT251450.017.432172
CGACGAG38250.017.234798
TCACGCT251850.016.9113588
TTCACGC263750.016.276427
GTCCGTT42750.015.5364691
AGACCGC48850.015.1085724
CGGCTTC322550.014.7336733
ACGCTCA293350.014.67231710
CCCGTAT32500.014.4094161
ACGCTAC294550.014.13131424
AACGCTA295000.014.11738623
CACGCTC308350.013.9585689
CGCTACC303100.013.73269125
CGAACGA36100.013.649343532
ACGAACG38350.013.61123431
Produced by FastQC (version 0.11.5)