FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47537263
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG157850.012.88428338
GGGAGGT203450.012.6050153
AGCTTAG158900.012.55801745
GGCATAG165250.012.116919535
GTCACTC210100.011.3419248
GGAGGTC228050.011.1663144
GGTCACT214550.011.1178237
GTTGTCG25500.010.93970426
CATAGGG185750.010.87626637
GAGCTTA182850.010.86403544
ACTCTCC231100.010.51491811
GCATAGG192500.010.50685336
TGGCATA197350.010.24864134
GAGGTCA254550.09.9684415
TAATCGG36350.09.6626784
CACCGAA264400.09.61475745
TCACTCT258350.09.5103959
CACTCTC256700.09.29976710
GACGCAA56250.09.2787844
TAGGGGA260400.09.08890539
Produced by FastQC (version 0.11.5)