FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49775398
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT553870.11127384656974516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG28450.024.040936
ACCGCGT128450.022.3149176
GACCGCG128450.022.2448545
AGACCGC138400.021.0032444
CCGCGTT139200.020.640097
TCGACGA34450.020.310997
CAGACCG146650.020.005783
CGCGTTC145450.019.7067818
GTTCGAC40050.017.6395345
CGACGAG38800.017.3960048
CGGTATA56650.017.3953920
GAACGAT175250.016.38162445
GCGTTCT184300.015.7113389
TCAGACC194850.015.6573872
AACGCTA136950.014.80207923
CCCGTAT34000.014.7881231
CTCAGAC247850.014.646171
ATACGGA198400.014.64038133
AATACGG197550.014.6350432
ACGCTAC138500.014.58768824
Produced by FastQC (version 0.11.5)