FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49775398
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG39450.014.59911526
ATAGGGG125050.014.45514638
GGCATAG129900.013.27085735
AGCTTAG136200.013.00153145
GGGAGGT212700.011.91013
CATAGGG147550.011.79317937
GGAGGTC210100.011.6505514
GGTCACT198050.011.6432757
ATATAGC61000.011.4329096
GCATAGG153700.011.320559536
GAGCTTA164100.010.99697244
GTCACTC215650.010.9015378
TGGCATA163100.010.85679134
AGGTCAC229950.010.5269786
GAGGTCA246750.010.3118565
GCTAAGT56400.010.29090814
TATAGCT69250.09.9084627
ACTCTCC237850.09.73264711
TCGTTCC64500.09.13891930
TAGCGGG20500.09.11178735
Produced by FastQC (version 0.11.5)