FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49443450
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG521260.10542549114190049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAG170450.015.59241135
AGCTTAG173250.015.38327845
ATAGGGG190100.014.32409338
GGTCACT253150.014.2376987
GTCACTC256000.014.20295058
CATAGGG200250.013.54209737
GAGCTTA199800.013.485085544
GGGAGGT304450.013.2394833
TGGCATA204500.013.05118734
ACTCTCC285450.012.96732611
GCATAGG209000.012.94257736
AGGTCAC289150.012.8673276
GGAGGTC301700.012.5111994
GAGGTCA311900.012.2178795
ACCCGGC86050.011.9661975
TAGGGGA319200.011.3007539
CACTCTC325350.011.22755910
TCACTCT336650.011.1802239
GTTGTCG27700.010.88564526
TCAGACG83200.010.8289312
Produced by FastQC (version 0.11.5)