FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53594582
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG815220.15210865904318463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT106400.020.294996
AGACCGC106100.020.2842964
GACCGCG109000.019.889165
TCACGCT265750.018.8497778
TTCGACG22000.018.506116
CCGGCTT290400.018.4001222
GCCGGCT261700.018.0108431
CCGCGTT120400.017.8416967
TTCACGC287300.017.5924647
CAGACCG127500.017.49893
AACGCTA277200.017.4950823
ACGCTAC278600.017.38294624
CGCTACC283600.017.02095625
ACGCTCA303400.016.71081410
CGGCTTC334100.016.5195453
CACGCTC308050.016.3490359
GTCCGTT43600.016.309151
ACGTTCG16950.016.1900946
TCGTATG34500.016.10415543
AAAACGC303450.016.10025621
Produced by FastQC (version 0.11.5)