FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52636589
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA133750.019.76583523
TTCGACG26200.019.7517836
ACGCTAC136700.019.32282824
CGCTACC143000.018.36140425
AAAACGC152950.017.3581921
TCACGCT172100.016.3548858
GAAAACG166350.015.97346120
TCGACGA33400.015.8977857
TTCACGC185100.015.6316797
AAACGCT172100.015.53128722
ACGCTCA192750.014.95292910
CGGTATA43150.014.80844420
CTACACG48600.014.44407845
GTCCGTT39850.014.2944221
GTTTTCG199500.013.375645
CGTGGTT202500.013.33315941
CACGCTC213250.013.3150079
CGACGAG36300.013.2021318
GTCCGAT27950.013.0499131
GACCGCG55700.012.8057825
Produced by FastQC (version 0.11.5)