FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52636589
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG122300.010.76133438
AGCTTAG127300.010.674170545
GGCATAG130000.09.95099735
GTCACTC188200.09.2660088
CATAGGG145300.09.11976237
TCTATAG180950.09.0208613
GGTCACT194200.08.922477
GAGCTTA153300.08.81984244
GTTGTCG24900.08.76392726
GCATAGG151400.08.693048536
CTATAGT189450.08.6280094
TGGCATA154250.08.57618634
CACCGAA191050.08.56077345
ATAGTGT205750.08.4767676
GGGAGGT239500.08.4136823
TTACTAG195450.08.36880614
GCACCGA198150.08.26543644
TACTAGA200450.08.22740215
TATAGTG205400.08.2101285
CGTAACG13750.08.18082342
Produced by FastQC (version 0.11.5)