FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49901866
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT766730.1536475609950137No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC595650.11936427387304516No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG549250.11006602438473943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG31600.024.421616
CGCTACC153000.021.6467325
AACGCTA271100.021.44563923
AAAACGC285750.020.3461521
ACGCTAC241050.020.26416224
TCGACGA39650.019.5201237
GAAAACG299250.019.43579920
AGACCGC110100.019.2296664
CGCTATC49800.019.11115825
TCACGCT310250.018.9929648
AAACGCT307500.018.97288722
GTTTTCG295500.018.86008345
CGTGGTT309750.018.5662741
CGGTATA54050.018.56586320
TCGTGGT328250.017.65695440
GTTAGTT225050.017.40583444
CGACGAG43300.017.3551188
CAGACCG124100.017.26013
TTCGGCG183300.017.02507644
ACGCTCA359050.016.55159810
Produced by FastQC (version 0.11.5)