FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42074845
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG1197330.2845714583143444No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA801730.1905485332150362No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT685480.1629191979198022No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG467220.11104497235818694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC149100.023.3411754
GACCGCG162650.021.3967045
CAGACCG171700.020.635953
ACCGCGT176050.019.818816
TCAGACC202500.018.119762
TTCGGCG84550.017.03459544
TCGGCGC87850.016.62454645
CTCAGAC262750.015.9965491
CGGCAGT398150.015.81681740
AGTTGCA413900.015.14241844
CGTACGC97450.014.9359575
AACGCTA123350.014.88204623
CCGCGTT276800.014.8431761
CCCGCGT21100.014.8226031
ACGCTAC124850.014.68550624
CCGTACG99350.014.6729424
GACGGCA429400.014.65035738
CTACACG33850.014.49254645
TGACGGC440500.014.2861837
TTCGCGC21200.014.2190237
Produced by FastQC (version 0.11.5)