FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48819353
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT713090.14606707303146765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC146800.023.817714
ACCGCGT156000.022.5283226
GACCGCG156800.022.3417055
CAGACCG161900.021.8325483
TTCGACG19700.020.6721066
CCGCGTT171000.020.4995237
CGCGTTC183850.019.0789688
GAACGAT186050.018.62414645
TCAGACC194750.018.5542772
ATACGGA194750.017.87326633
AATACGG195150.017.83637632
TCACGCT203650.016.715798
TACGGAG210150.016.58497834
GTTAGTT124050.016.39671944
CAATACG215900.016.27845831
AACGCTA203300.016.11374323
ACGCTAC203800.016.08524924
AGAACGA217300.016.04934344
TCGACGA27100.015.6915177
CTACACG69450.015.64804845
Produced by FastQC (version 0.11.5)