FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48819353
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG787270.1612618667846745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG233300.016.98190945
GGCATAG240900.016.39961435
ATAGGGG256650.015.428431538
GTCACTC368150.015.1755848
GTTGTCG26750.015.05457426
GGGAGGT395900.015.0389633
GGTCACT375700.014.7994487
TGGCATA268550.014.70263634
ACTCTCC378400.014.6923611
GAGCTTA272800.014.63850844
GGAGGTC389400.014.5382974
GAGGTCA413450.013.9301865
CATAGGG289500.013.66995937
AGGTCAC423000.013.644836
GCATAGG307600.012.90208636
TAGCGTA11350.012.88449537
TCACTCT443700.012.7993039
CACTCTC436000.012.78784110
TGGGAGG341750.012.3969432
TAGGGGA454000.012.0767539
Produced by FastQC (version 0.11.5)