FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51239486
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT588430.1148391691516968No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG573850.11199370735295823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG20450.020.023966
ACCGCGT148450.019.4757796
AGACCGC149900.019.2723774
GACCGCG150400.019.1634275
CCGCGTT161600.017.8770567
CAGACCG163800.017.8154983
CGCGTTC170700.016.9635728
ACGTTCG17900.016.8431636
GAACGAT177400.016.31019845
TCACGCT205400.015.4560628
CGTACGC56800.015.4485655
CCGTACG58250.015.2185124
TCAGACC198300.015.158482
TCGACGA27500.015.1360087
ATGCGCG35150.014.85027744
TGCGCGT35300.014.72343545
GTCCGTT38100.014.59853651
TCGGCGC58200.014.49706745
AACGCTA211300.014.39623923
ACGCTAC212050.014.33471224
Produced by FastQC (version 0.11.5)