FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51239486
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3873890.7560360773330161No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG529690.1033753539213879No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG186750.015.09742345
GTTGTCG31100.014.32423226
GGCATAG203800.013.80122935
GTCACTC305200.013.3142558
GGTCACT301350.013.2684327
GAGCTTA218850.013.0162644
ATAGGGG218600.012.90493438
GGGAGGT342450.012.7399363
GGAGGTC324900.012.5695314
TGGCATA228400.012.36368634
ACTCTCC329700.012.29692211
AGGTCAC343450.012.048166
ACCCGGC91300.011.9034975
TCACCCG94200.011.8472463
GAGGTCA354550.011.8421995
TAGCGTA11650.011.39277137
CATAGGG252000.011.14082437
TCACTCT393000.010.7506979
CGGACAG100250.010.72941219
GCATAGG265950.010.64184336
Produced by FastQC (version 0.11.5)