FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52390318
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA864540.16501904035016546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG16800.018.2124086
AACGCTA117400.016.71059623
ACGCTAC106950.016.49449224
CGCGTGT10800.016.0716111
TAGTTAT168600.015.93358838
CGCTACC111100.015.75684425
CCCGTAT29300.015.6947721
AGTTATA175750.015.32385639
CTCCGAT44650.015.2467721
GTTATAT162900.015.19309440
TCGTTAC19900.015.15031944
GACATAG180000.015.13793534
GCCGGCT143900.015.0384441
GTCAACG73350.014.90904229
CCGGCTT151000.014.6607412
AGACATA195850.014.49886733
CTCGTCT164900.014.4492991
ATAGTTA186850.014.37767937
AAAACGC139550.014.33209521
TTCGCGC9500.014.2090637
Produced by FastQC (version 0.11.5)