FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56695489
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG30050.021.1846736
TCGACGA37400.017.3219227
CGGTATA51350.016.4710720
AACGCTA111850.016.37053523
ACCGCGT97100.016.123896
AGACCGC97500.016.0577264
ACGCTAC114250.016.02664624
GAACGAT99650.015.875771545
CGACGAG40850.015.4184678
GACCGCG103100.015.2946375
CGCTACC125000.014.84630525
CAGACCG110650.014.4339753
CCGCGTT109200.014.29591757
AAAACGC132250.014.066442521
CGCGTTC112000.013.9586048
TTCGGCG81450.013.61550444
GTCCGTT44600.013.4709541
ACGGTAC73400.013.47088245
TCGGCGC81950.013.3135945
CCCGTAT38800.013.2228491
Produced by FastQC (version 0.11.5)