FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56695489
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4433470.7819793211414051No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC100750.011.729565
AGCTTAG108450.010.32067945
GGCATAG106850.010.26419335
TCACCCG123250.010.2596563
CGGACAC117250.010.23281813
CATAGGG111250.010.05863237
ACGGACA121650.010.00604318
ATAGCTC116600.09.86514139
CACCCGG124000.09.8366214
CCGGACA125900.09.5842712
GCATAGG115350.09.43044736
CGGACAG124900.09.40604619
ACACGGA128100.09.328794516
TAGCGTA13550.09.23102537
ATAGGGG125350.09.15864938
GACACGG130500.09.12314415
GTCACTC176200.09.0884848
CACCGAA173150.08.98021745
TAGCTCT131900.08.83947640
GCACCGA175250.08.82113744
Produced by FastQC (version 0.11.5)