FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53226423
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG30450.015.0724326
AGCTTAG143800.014.75780445
GGCATAG154450.013.67940135
TGTCGTT37000.012.58644128
GAGCTTA172800.012.38527744
ATAGGGG174950.012.37251138
CATAGGG175100.012.13051637
TCACCCG101800.011.8047263
TGGCATA182150.011.710093534
ACGGACA96600.011.69226918
GCATAGG184900.011.42662736
ATAGCTC96450.011.0116439
GGGAGGT308600.010.8801613
TATAGCT52450.010.8098677
ACCCGGC108500.010.6817295
TAGGGGA269900.010.51294639
GCTAAGT45100.010.42677914
CGGACAC106450.010.42018713
GTCAGGA218300.010.391041
TAGCGTA11750.010.34068637
Produced by FastQC (version 0.11.5)