FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43325284
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG25350.021.7449936
TCGACGA29750.018.3020447
CGGTATA38050.017.79846220
CGACGAG30550.016.8653348
GTCCGTT37150.015.9374111
CCGTACG63950.015.6227574
AACGCTA92700.015.58209823
CTCGGTT92700.015.4210811
ACGCTAC94350.015.28575124
CGTACGC66200.015.1937435
TCGGCGC65350.014.97665545
TTCGGCG65950.014.66982244
CGCTACC99350.014.448523525
CTACACG45250.014.3697945
GTCCGAT25950.013.96721
CTCCGTT69550.013.8537531
CGCCCCG71950.013.75917923
TACGCCA73950.013.721757
TCCGTAC76450.013.6864473
CGATTAG24150.013.603514
Produced by FastQC (version 0.11.5)