FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48331186
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG638750.13216104400996906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG27200.025.6347876
TCGTATG34850.021.88769745
TCGACGA32750.021.6339667
CGTCCCG37450.021.27833435
ACCCGTC36550.020.7476632
ACCGCGT106500.019.9792166
AGACCGC108800.019.5568624
CGGTATA50800.018.86282220
GACCGCG114100.018.6878625
CCGCGTT116800.018.2366097
CGACGAG38750.017.9359598
CAGACCG123250.017.574233
CGCGTTC128350.016.6481348
ACGTTCG24200.015.9863756
TCGGCGC69400.015.85450245
TCACGCT237300.015.8196348
TGCGCGT28650.015.55047945
AACGCTA233300.015.46632823
ATGCGCG29150.015.36795144
ACGCTAC235650.015.32166924
Produced by FastQC (version 0.11.5)