FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48331186
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG99200.016.3954723
GGTCGCC40250.016.09992443
ACGGACA102900.015.41747618
ACCCGGC105750.014.993815
CGGACAC105400.014.96821713
GTTGTCG25100.014.34461726
CACCCGG116550.013.9710664
CCGGACA123200.013.2780412
AGCTTAG167800.013.254312545
ACACGGA119150.013.1468416
CACGGAC126450.012.52864717
GACACGG124150.012.5268415
TCTCGGT57950.012.38690636
AGTGTAG57300.012.29285228
GCCCGGA132750.012.28268310
GGCATAG180100.012.24574635
ATAGCTC122700.012.13787639
TCAGACG101500.011.748292
CGGCCCG140850.011.5263278
GTAGGGA60750.011.37186318
Produced by FastQC (version 0.11.5)