FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46546092
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1380090.29649965028213326No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG963020.20689599461969865No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT614140.1319423336335089No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA600570.12902694387318273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG42900.028.9439016
GTTCGAC49450.025.5215385
TCGACGA53800.023.5815377
CGACGAG55250.022.596288
AGACCGC319900.022.1446464
CGGTATA76050.021.56320
GACCGCG327600.021.555485
ACCGCGT329400.021.5247386
CAGACCG336200.021.1579913
CCGCGTT350800.020.218077
CGCGTTC360650.019.6783948
AGTTCGA65100.019.5935154
TCAGACC378100.019.3250062
TCACGCT400850.018.8104138
CCGGCTT374600.018.7428782
ATGACGG83000.018.59245516
GCGTTCT391600.018.1690739
TTCACGC417400.018.1669337
CTCAGAC480600.017.8128171
GAACGAT390700.017.69634645
Produced by FastQC (version 0.11.5)