FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48984762
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC92000.023.6021294
ACCGCGT94450.022.9658166
GACCGCG97950.022.1913855
CCGCGTT105200.020.617137
CGCGTTC98800.019.789278
CAGACCG118050.018.6226373
TTCGACG17550.016.795816
ACGCTAC140200.016.60999524
AACGCTA140600.016.51478223
CGCTACC120750.016.06209425
CCCGTAT28250.015.5345081
TCAGACC151550.014.8945122
TTCGGCG68700.014.47620244
AAAACGC163450.014.37118921
CTCAGAC205350.014.1485811
GTTCCTA77250.014.03834515
GAAAACG157350.013.99887320
CCGGTAT49600.013.8388331
GCCGGCT175900.013.817811
GCGTTCT143200.013.7948969
Produced by FastQC (version 0.11.5)