FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48984762
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG500730.10222158474506828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAG166100.015.15696635
AGCTTAG168350.015.08604745
ATAGGGG173600.014.69607838
GGGAGGT273900.014.168573
GGAGGTC261850.013.9088644
GTTGTCG26650.013.84504926
TGGCATA192500.013.29950434
GAGCTTA192700.013.2386944
CATAGGG193350.013.21856337
GGTCACT271250.012.9826267
ACTCTCC282100.012.68187811
GTCACTC281000.012.6362378
GCATAGG205300.012.51494736
TAGGGGA306350.011.31683839
GAGGTCA335050.011.0103215
GCGTAGG86850.010.8800636
AGGTCAC342350.010.8055266
GGCGTAG88550.010.59496135
CGTAGGG89350.010.52523537
CACTCTC347700.010.50285510
Produced by FastQC (version 0.11.5)