FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50562332
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG124200.014.43489445
GGCATAG136250.013.20844735
ATAGGGG146000.012.83430738
CATAGGG146000.012.31068937
GAGCTTA154700.011.92340844
TAGCGTA8050.011.73658837
GCATAGG156000.011.63701636
GGGAGGT224600.010.9015973
TGGCATA167100.010.89116334
GTCACTC207800.010.6789298
TCGCGCT32300.010.51601745
ACCCGGC77550.010.4182515
GGAGGTC219800.010.2491674
ACTCTCC218050.010.07369511
TAGGGGA212150.010.01995139
TAATCGG32650.09.9257084
GGTCACT223450.09.9208487
TCACCCG92050.09.5837013
ACGCAAG66000.09.40680245
GACGCAA64600.09.36691644
Produced by FastQC (version 0.11.5)