FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46523965
Sequences flagged as poor quality0
Sequence length51-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACGC23100.040.23353664-65
CTACGCC25400.036.58951666-67
CCCCTAT74700.033.24957762-63
CTATGCC80050.032.92886764-65
GCCCTAA77650.032.40299252-53
CCCTAAG87850.031.4683752-53
CCCTACG31150.029.83610564-65
CCTAAGC87700.029.14538454-55
CTAAGCT95700.028.38353354-55
CCCTATG94000.028.2127362-63
TATGCCG33200.026.06051394-95
ACACTAT85650.025.90894386-87
CTAGTAA90000.025.10271878-79
AGCCCTA122050.025.08100750-51
CCTATGC98250.025.03502764-65
TAAGCTC102650.024.90079556-57
GCCGGCT207750.024.8546941
ACTATAG88750.024.5526288-89
TCACGCT222900.024.275568
CCGGCTT243500.024.149442
Produced by FastQC (version 0.11.5)