FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46523965
Sequences flagged as poor quality0
Sequence length51-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA31900.026.3701794-95
GTAGGGA563650.023.38140994-95
TCGTGTA744100.023.09459394-95
GTCGTGT810650.023.00720694-95
ACGTTGA121900.022.93645186-87
AGCGTCG1023550.022.68316594-95
TGTAGGG623750.022.68268694-95
CACAACG146300.022.61651282-83
CGTCGTG867300.022.57595894-95
TAGGGAA576650.022.27078494-95
GCGTCGT931100.021.85504594-95
GTAGATC228850.021.42470494-95
CAACGTT156750.020.95480384-85
AGAGCGT1219850.020.56321194-95
GCGTCGG33200.020.51139594-95
AAGAGCG1340250.020.34782694-95
GTGTAGG753600.019.84804594-95
CGTTGAA122500.019.42338286-87
AGTGTAG267500.018.41898294-95
GAGCGTC1345550.018.41596294-95
Produced by FastQC (version 0.11.5)