FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55984408
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG875530.15638818579630243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT389450.015.7778413
GGAGGTC377900.015.676164
AGCTTAG312600.015.07177345
GTTGTCG53000.014.595942526
GGCATAG324000.014.39363735
ATAGGGG332400.014.37876938
GAGGTCA420550.014.2843355
CACCGAA363850.014.08720345
AGGTCAC425600.013.9946176
GGTCACT420550.013.7932847
GTCACTC425200.013.5943698
ATATAGC62100.013.5566646
GCACCGA377800.013.442039544
GAGCTTA350500.013.43574244
TGGCATA360500.012.99876834
TGGGAGG383600.012.9896142
ACTCTCC448850.012.98901211
TATAGCT65500.012.9559997
GCTAAGT62500.012.63238914
GCGTCGT35300.012.047502511
Produced by FastQC (version 0.11.5)